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Videos DIA/SWATH Course 2017

Day 1: Biological significance and introduction to SWATH/DIA

Ben Collins - Opening remarks

Ruedi Aebersold - Some considerations of DIA/SWATH in experimental biology

Ben Collins - Introduction to DIA/SWATH acquisition and concepts

Olga Vitek - Introduction to experimental design and statistical analysis

Ben Collins - Generating Libraries for DIA/SWATH analysis

Day 2: Concepts of peptide-centric analysis

Brendan MacLean - SkyJam 1

Michael MacCoss - Introduction to peptide-centric analysis and DIA on trapping instruments

Ludovic Gillet - Concepts of peptide-centric DIA analysis - Automated peak picking and false discovery rates

Isabell Bludau - FDR control in the DIA workflow and TRIC alignment

Ludovic Gillet - Acquisition methods on Q-TOF and Q-Orbitrap

Brendan MacLean - Introduction to Skyline for DIA analysis

Brendan MacLean - Skyline DIA examples

Day 3: Spectrum-centric analysis, quantification and statistics

Brendan MacLean - SkyJam 2

Alexey Nesvizhskii - Introduction to spectrum-centric analysis of DIA data

Alexey Nesvizhskii - Using DIA-UMPIRE and related tools

Olga Vitek - Statistical analysis of DIA data

Tina Ludwig - Quantitative concepts and absolute label free quantification

Day 4: Hands-on analysis and future developments

Brendan MacLean - SkyJam 3

Michael MacCoss - Recent developments and future direction in DIA

Skyline Session: Visualizing external data in Skyline - Brendan MacLean

Day 5: Post-translational modifications and DIA applications

Brendan MacLean - SkyJam 4

Tina Ludwig - Concepts of PTM analyses using DIA

George Rosenberger - Inference of peptidoforms

Lukas Reiter - Tiered iRT: Making the best use of multiple retention time spaces for DIA

Ruedi Aebersold - Biological applications of SWATH/DIA MS

Videos DIA/SWATH Course 2017

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