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DIA/SWATH Course

Videos DIA/SWATH Course 2017


Day 1: Biological significance and introduction to SWATH/DIA


Ben Collins - Opening remarks


Ruedi Aebersold - Biological significance of SWATH-MS experiments


Christina Ludwig - Targeted Proteomics Primer


Ben Collins - Introduction to DIA/SWATH acquisition and concepts


Christina Ludwig - General principles of quantitative proteomics


Eduard Sabidó - Quality control in MS-based proteomics


Ben Collins - Generating Libraries for DIA/SWATH analysis


Day 2: Concepts of peptide-centric analysis


Brendan MacLean - SkyJam 1


Michael MacCoss - Introduction to DIA on Orbitraps and peptide-centric versus spectrum-centric analysis


Ludovic Gillet - Concepts of peptide-centric DIA analysis - Automated peak picking and false discovery rates


Isabell Bludau - Details on FDR control in a DIA workflow and TRIC


Brendan MacLean - Introduction to Skyline for DIA analysis


Day 3: Concepts of spectrum-centric analysis


Brendan MacLean - SkyJam 2


Alexey Nesvizhskii - Introduction to spectrum-centric analysis of DIA data


Olga Vitek - Introduction to experimental design and statistical analysis


Alexey Nesvizhskii - Details on DIA-UMPIRE


Skyline Walk-Through - Brendan MacLean


Day 4: Data analysis in comparison


Brendan MacLean - SkyJam 3


Michael MacCoss - Recent developments and furture direction in DIA


Ariel Bensimon - Analysis of post-translational modifications by SWATH-MS


Day 5: Downstream analysis and application


Brendan MacLean - SkyJam 4


Olga Vitek - Statistical analysis of DIA data


Lukas Reiter - An industrial perspective on DIA and the Spectronaut sofftware


Ruedi Aebersold - Reproducibility of research results in proteomics


Videos Targeted Proteomics Course 2016


Videos Targeted Proteomics Course 2015