DIA logo

DIA/SWATH Course

Videos DIA/SWATH Course 2017


Day 1: Biological significance and introduction to SWATH/DIA


Ben Collins - Opening remarks


Ruedi Aebersold - Some considerations of DIA/SWATH in experimental biology


Ben Collins - Introduction to DIA/SWATH acquisition and concepts


Olga Vitek - Introduction to experimental design and statistical analysis


Ben Collins - Generating Libraries for DIA/SWATH analysis


Day 2: Concepts of peptide-centric analysis


Brendan MacLean - SkyJam 1


Michael MacCoss - Introduction to peptide-centric analysis and DIA on trapping instruments


Ludovic Gillet - Concepts of peptide-centric DIA analysis - Automated peak picking and false discovery rates


Isabell Bludau - FDR control in the DIA workflow and TRIC alignment


Ludovic Gillet - Acquisition methods on Q-TOF and Q-Orbitrap


Brendan MacLean - Introduction to Skyline for DIA analysis


Brendan MacLean - Skyline DIA examples


Day 3: Spectrum-centric analysis, quantification and statistics


Brendan MacLean - SkyJam 2


Alexey Nesvizhskii - Introduction to spectrum-centric analysis of DIA data


Alexey Nesvizhskii - Using DIA-UMPIRE and related tools


Olga Vitek - Statistical analysis of DIA data


Tina Ludwig - Quantitative concepts and absolute label free quantification


Day 4: Hands-on analysis and future developments


Brendan MacLean - SkyJam 3


Michael MacCoss - Recent developments and future direction in DIA


Skyline Session: Visualizing external data in Skyline - Brendan MacLean


Day 5: Post-translational modifications and DIA applications


Brendan MacLean - SkyJam 4


Tina Ludwig - Concepts of PTM analyses using DIA


George Rosenberger - Inference of peptidoforms


Lukas Reiter - Tiered iRT: Making the best use of multiple retention time spaces for DIA


Ruedi Aebersold - Biological applications of SWATH/DIA MS


Videos DIA/SWATH Course 2017


Videos Targeted Proteomics Course 2016


Videos Targeted Proteomics Course 2015